Isotopes in Experimental Pharmacology

The latest volume in this valuable series includes the following chapters: (1) The Determination of Amino Acids by High-Voltage Paper Electrophoresis, by S. Blackburn, who gives useful details for the rapid separation and analysis of amino acid mixtures; this reviewer was not convinced, however, that present-day electrophoretic methods can match ion-exchange chromatography in precision. (2) Mobility Determination by Zone Electrophoresis at Constant Current, bv H. Waldmann-Meyer. This chapter will be valuable to those wishing to determine the electrophoretic mobility of proteins. (3) Methods for the Study of Reversible Denaturation of Proteins and Interpretation of Data, by J. Hermans. (4) The Use of X-ray Diffraction in the Study of Protein and Nucleic Acid Structure, by K. C. Holmes and D. M. Blow. This chapter represents an excellent introduction to the most important experimental method now available for the definition of the three-dimensional structure of macromolecules, and is highly recommended. (5) The Determination of Nitrogen in Biological Materials, by S. Jacobs. (6) Enzymatic Methods Used for Diagnosis, by E. Amador and W. E. C. Wacker. This chapter provides a valuable compilation of methods that have proved to be useful in enzyme assays for routine diagnostic purposes. (7) Applications of the RM Treatment in Chromatographic Analysis, by I. E. Bush, who offers an excellent discussion of the theory of partition chromatography developed by A. J. P. Martin and his colleagues. This chapter will be valuable to all workers who wish to correlate chemical structure with chromatographic behavior.